Fig 1: Normal skin, dog. Round cells with a typical mast cell morphology in the dermis are immunolabeled for both c-kit (a) and CPA3 (b). CPA3 labels the cytoplasm diffusely and obscures the nucleus. Mast cells are close to a dermal blood vessel (*). Inset: Higher magnification of a labeled cell. Figure 2. Normal small intestine, dog. Numerous c-kit (a) and CPA3 (b) positive cells are present in the lamina propria. The cytoplasm of mast cells is diffusely labeled for CPA3. Mast cells are close to a mucosal blood vessel (*). Inset: Higher magnification of a labeled cell. Figure 3. Normal liver, dog. Mast cells in portal areas are immunolabeled for both c-kit (a) and CPA3 (b). CPA3 labels the mast cell cytoplasm diffusely, sometimes condensing to a more dotted pattern. Inset: Higher magnification of a labeled cell.
Fig 2: Low-grade MCT, skin, dog. Diffuse cytoplasmic CPA3 labeling pattern (arrows) is characterized by evenly stained cytoplasm, sometimes obscuring the nucleus. A membranous pattern is also present. Figure 9. High-grade MCT, skin dog. The focal cytoplasmic CPA3 labeling pattern (arrows) is characterized by focal intensively labeled stipples, spots, or dots in the cytoplasm. The cytoplasmic CPA3-positive aggregates vary in size, and in these cells the nucleus is often eccentric. Figure 10. High-grade MCT, skin, dog. Neoplastic cells in the main tumor cell mass (black asterisk) label weakly or moderately for CPA3 with mostly focal cytoplasmic labeling pattern. The infiltrating mast cells (arrow heads) label more intensely and diffusely for CPA3.
Fig 3: Normal spleen, dog. Both c-kit (a) and CPA3 (b) positive cells are in the white pulp. Inset: higher magnification of a labeled cell. Figure 5. High-grade MCT, skin, dog. Similar cells are labeled with antibodies to c-kit (a) and to CPA3 (b). Figure 6. Low-grade MCT, skin, dog. Membranous CPA3 labeling pattern (arrows) is characterized by membrane-associated labeling with weak or no labeling in the cytoplasm surrounding the nucleus. Figure 7. High-grade MCT, skin, dog. Membrane-associated labeling (arrows) is present in neoplastic cells, including within the multinucleated neoplastic cell.
Fig 4: Using qPCR, mRNA expression levels of DEGs were determined in a validation cohort of achalasia. (a) CPA3 is enriched in the esophageal mucosa of achalasia subjects in distal esophagus. *P < 0.02. (b) IL-33 mRNA expression levels are increased in the proximal esophagus of achalasia subjects. **P < 0.005. (c) Increased IFNε is seen in both proximal and distal esophageal achalasia. **P < 0.002, ***P < 0.001. (d) The proximal and distal esophageal mucosa of achalasia is enriched for CDH16. *P < 0.05, ***P < 0.001. (e) Decreased MAMDC2 is observed in the proximal esophageal mucosa of achalasia. *P < 0.05. n = 4 healthy control and 23 achalasia.
Fig 5: Heterogeneity of MCs in CRC.a. UMAP plots of 4,155 MCs colored by cluster (left) and tissue type (center) in the GSE178341 dataset. Bar charts show the proportion of MC subtypes in different tissues (right). b. UMAP plots of MC activated signature. c. Log ratio of average fraction per MC clusters in tumor to normal tissue (top). Mann-Whitney U test, *: p < 0.05, **: p < 0.01, ***: p < 0.001. Dot plots of cytokine and growth factor, protease and histamine, lipid mediator and various receptor-related gene expression in MC clusters (bottom). d. Volcano plot of differentially expressed genes between resting and activated MCs. e. Differential pathway enriched in resting and activated MCs by GSVA, showing the top 10 significant enriched hallmark terms. f. Differentiation trajectory of MCs with each color coded for MC subsets (left), tissue types (center), and pseudotime (right). g. Pseudotime trajectory of CMA1, CPA3, TPSAB1, and KIT expression levels
Supplier Page from MilliporeSigma for Anti-CPA3 antibody produced in rabbit